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Image Search Results
Journal: Frontiers in Oncology
Article Title: Monocyte Chemoattractant Protein 1 Promotes VEGF-A Expression in OSCC by Activating ILK and MEK1/2 Signaling and Downregulating miR-29c
doi: 10.3389/fonc.2020.592415
Figure Lengend Snippet: Clinical significance of MCP-1 in angiogenesis of oral squamous cell carcinoma. (A–C) OSCC tissue and adjacent normal tissue data from the Oncomine database ( https://www.oncomine.org ) were analyzed for MCP-1, VEGF-A, and ANGPT2 expression. (D) IHC photographs of tissue array sections (OR601) immunostained with anti-MCP-1 or anti-VEGF-A antibody. (E, F) IHC results were scored on a scale of 1–5 for staining intensity. Levels of MCP-1 and VEGF-A expression correlated with OSCC clinical grade. **p < 0.005 , ***p < 0.0005 compared with controls. (A–C, E) are box-and-whisker plots that plot outliers as individual points (dots).
Article Snippet: The
Techniques: Expressing, Staining, Whisker Assay
Journal: Frontiers in Oncology
Article Title: Monocyte Chemoattractant Protein 1 Promotes VEGF-A Expression in OSCC by Activating ILK and MEK1/2 Signaling and Downregulating miR-29c
doi: 10.3389/fonc.2020.592415
Figure Lengend Snippet: MCP-1 promotes human OSCC cell angiogenesis through the VEGF-A-dependent pathway. Cells were incubated with MCP-1 (0–50 ng/ml) for 24 h and VEGF-A expression was determined by Western blot (A) , RT-qPCR (B) , and ELISA assays (C) . The CM was collected and used to treat the EPCs for 24 h. We used the tube formation assay to examine capillary-like structure formation (D) and the Transwell assay to examine cell migration (E) . * p < 0.05 compared with controls; # p < 0.05 compared with MCP-1–treated controls. Each experimental procedure was independently repeated three times, with similar results.
Article Snippet: The
Techniques: Incubation, Expressing, Western Blot, Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay, Tube Formation Assay, Transwell Assay, Migration